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81.
In this work, gliadin composition has been analyzed in 33 accessions of durum wheat using MALDI-TOF MS and compared with A-PAGE results. The MALDI-TOF MS spectra were 29,900-42,500 Da, which corresponds to the α/β- and γ-gliadin regions in A-PAGE. The average of gliadin peaks per line was 23 for MALDI-TOF MS and only 14.8 bands for A-PAGE. MALDI-TOF MS identified 33 gliadin peaks in the durum wheat collection, 20 of which were unique peaks present in 7 lines. A-PAGE analysis identified 30 bands, of which only 4 were unique. Thus, the MALDI-TOF MS method was more sensitive than A-PAGE for identifying α/β- and γ-gliadins in the 33 durum wheat lines studied. Phylogenetic analyses performed using MALDI-TOF MS data assigned the durum wheat lines to two groups. The utility of MALDI-TOF MS to determine relationships among genotypes and for identification of durum wheat accessions is discussed. 相似文献
82.
In this study a gene for a drought stress-inducible putative membrane protein was cloned and characterised from root tissue of wild emmer wheat. Sequence analysis indicated that the protein is a member of the widespread but hitherto uncharacterised TMPIT (transmembrane protein inducible by TNF-α) family, so it was labelled TdicTMPIT1. Real-time RT-PCR showed that the TdicTMPIT1 gene is upregulated on drought stress in drought-tolerant wild emmer wheat, but not in a drought-sensitive accession or in cultivated durum wheat. The TdicTMPIT1 product was predicted to be a membrane protein with four transmembrane helices. The protein was expressed and analysed in Escherichia coli and Saccharomyces cerevisiae. Cellular localisation of the protein in the cell was also investigated using an eGFP-tagged form of the protein in S. cerevisiae. Results obtained by confocal laser microscopy indicated that the TdicTMPIT1 tagged with GFP was localised in a membraneous compartment. It is concluded that TdicTMPIT1 is a membrane protein associated with the drought stress response in wild emmer wheat, and so it may be useful for the improvement of modern wheat genotypes. Members of this protein family in other organisms are proposed also to be involved in stress responses. 相似文献
83.
Ramirez-Garcia A Gallot N Abad A Mendoza L Rementeria A Hernando FL 《Applied microbiology and biotechnology》2011,92(1):133-145
Systemic candidiasis remains a major complication among patients suffering from hematological malignancies and favors the
development of hepatic metastasis. To contribute to the understanding of the underlying mechanisms, the aim of this study
was to identify molecules that may increase tumor cell adhesion to hepatic endothelial cells. To this end, a well-established
in vitro model was used to determine the enhancement of tumor cell adhesion induced by Candida albicans and its fractions. Different fractions were obtained according to their molecular weight (M
r) (five) or to their isoelectric point (pI) (four), using preparative electrophoresis and preparative isoelectric focusing, respectively, followed by affinity chromatography.
The fraction that most enhanced melanoma cell adhesion to endothelium had an M
r range from 45 to 66 kDa. It was characterized using two-dimensional electrophoresis, and 14 proteins were identified by peptide
mass fingerprinting: Dor14p, Fba1p, Pdi1p, Pgk1p, Idh2p, Mpg1p, Sfa1p, Ape3p, Ilv5p, Tuf1p, Act1p, Eno1p, Qcr2p, and Adh1p.
Of these, several are related to the immunogenic response, and the latter seven belonged to the most reactive fraction according
to their pI range, from 5 to 5.6. These findings could represent a step forward in the search for new targets, to suppress the pro-metastatic
effect of C. albicans. 相似文献
84.
Jones AK Rayes D Al-Diwani A Maynard TP Jones R Hernando G Buckingham SD Bouzat C Sattelle DB 《The Journal of biological chemistry》2011,286(4):2550-2558
The nematode Caenorhabditis elegans is an established model organism for studying neurobiology. UNC-63 is a C. elegans nicotinic acetylcholine receptor (nAChR) α-subunit. It is an essential component of the levamisole-sensitive muscle nAChR (L-nAChR) and therefore plays an important role in cholinergic transmission at the nematode neuromuscular junction. Here, we show that worms with the unc-63(x26) allele, with its αC151Y mutation disrupting the Cys-loop, have deficient muscle function reflected by impaired swimming (thrashing). Single-channel recordings from cultured muscle cells from the mutant strain showed a 100-fold reduced frequency of opening events and shorter channel openings of L-nAChRs compared with those of wild-type worms. Anti-UNC-63 antibody staining in both cultured adult muscle and embryonic cells showed that L-nAChRs were expressed at similar levels in the mutant and wild-type cells, suggesting that the functional changes in the receptor, rather than changes in expression, are the predominant effect of the mutation. The kinetic changes mimic those reported in patients with fast-channel congenital myasthenic syndromes. We show that pyridostigmine bromide and 3,4-diaminopyridine, which are drugs used to treat fast-channel congenital myasthenic syndromes, partially rescued the motility defect seen in unc-63(x26). The C. elegans unc-63(x26) mutant may therefore offer a useful model to assist in the development of therapies for syndromes produced by altered function of human nAChRs. 相似文献
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87.
Mattia Mazzoli Emanuele Pepe David Mateo Ciro Cattuto Laetitia Gauvin Paolo Bajardi Michele Tizzoni Alberto Hernando Sandro Meloni Jos J. Ramasco 《PLoS computational biology》2021,17(10)
Assessing the impact of mobility on epidemic spreading is of crucial importance for understanding the effect of policies like mass quarantines and selective re-openings. While many factors affect disease incidence at a local level, making it more or less homogeneous with respect to other areas, the importance of multi-seeding has often been overlooked. Multi-seeding occurs when several independent (non-clustered) infected individuals arrive at a susceptible population. This can lead to independent outbreaks that spark from distinct areas of the local contact (social) network. Such mechanism has the potential to boost incidence, making control efforts and contact tracing less effective. Here, through a modeling approach we show that the effect produced by the number of initial infections is non-linear on the incidence peak and peak time. When case importations are carried by mobility from an already infected area, this effect is further enhanced by the local demography and underlying mixing patterns: the impact of every seed is larger in smaller populations. Finally, both in the model simulations and the analysis, we show that a multi-seeding effect combined with mobility restrictions can explain the observed spatial heterogeneities in the first wave of COVID-19 incidence and mortality in five European countries. Our results allow us for identifying what we have called epidemic epicenter: an area that shapes incidence and mortality peaks in the entire country. The present work further clarifies the nonlinear effects that mobility can have on the evolution of an epidemic and highlight their relevance for epidemic control. 相似文献
88.
Oscar Hernando Hernández Jéssica Adriana Hernández-Sánchez José Daniel Flores-Gutiérrez 《Biological Rhythm Research》2018,49(3):405-411
Studies in temperate countries show a direct correlation between the hours of daily light and the frequency of suicides. This relationship is damped in countries at the equatorial level, and what happens near the tropic lines is unknown. In this paper the suicide rates as well as the monthly average of daily light were analyzed in hours from 1990 to 2016 in Campeche, Mexico, a place with a high rate of suicide deaths and near the latitude of the Tropic of Cancer. The results showed that total suicides and sunshine were positively correlated (r = 0.649; p = 0.022) and the peak of maximum light in June coincides with the maximum suicide peak. There were non-significant peaks of higher suicides in June and December for men and in May for women. However, a clear, significant peak (p = 0.003) showed up in spring. We recommend strengthening the prevention programs during these periods. 相似文献
89.
Microglial immune response is impaired against the neurotropic fungus Lomentospora prolificans 下载免费PDF全文
Aize Pellon Andoni Ramirez‐Garcia Xabier Guruceaga Alazne Zabala Idoia Buldain Aitziber Antoran Juan Anguita Aitor Rementeria Carlos Matute Fernando L. Hernando 《Cellular microbiology》2018,20(8)
Lomentospora (Scedosporium) prolificans is an opportunistic pathogen capable of causing invasive infections in immunocompromised patients. The fungus is able to disseminate via the bloodstream finally arriving at the central nervous system producing neurological symptoms and, in many cases, patient death. In this context, microglial cells, which are the resident immune cells in the central nervous system, may play an important role in these infections. However, this aspect of anti‐L. prolificans immunity has been poorly researched to date. Thus, the interactions and activity of microglial cells against L. prolificans were analysed, and the results show that there was a remarkable impairment in their performance regarding phagocytosis, the development of oxidative burst, and in the production of pro‐inflammatory cytokines, compared with macrophages. Interestingly, L. prolificans displays great growth also when challenged with immune cells, even when inside them. We also proved that microglial phagocytosis of the fungus is highly dependent on mannose receptor and especially on dectin‐1. Taken together, these data provide evidence for an impaired microglial response against L. prolificans and contribute to understanding the pathobiology of its neurotropism. 相似文献
90.
Fernando L. Hernando Esther Calvo Jose A. Rodriguez Pedro L. Barea Aitor Rementeria Maria Jesus Sevilla Jose Ponton 《Mycopathologia》1996,134(1):1-6
The effect of germ tube induction on the antigenic variability in C. albicans was studied in strains from blood cultures (Group I) and superficial candidiasis (Group II). When compared by immunoblotting with a rabbit antiserum, antigenic extracts from Group I strains grown as blastospores showed a higher reactivity than that of Group II strains. Major bands in Group I strains (45–47, 33, 30 kDa) were continuously expressed through the subcultures in vitro but, with the exception of the 45 kDa band, the reactivity of all of them decreased or disappeared after the tenth subculture in Group II strains. The induction of the germ tubes produced the re-expression of the antigens lost during subculture in the yeast form, the effect being very clear in Group II strains. The re-expression by C. albicans germ tubes of antigens lost during subculture of blastospores in vitro and the higher reactivity shown by Group I strains grown in mycelial phase should be taken into consideration when a test to detect anti-C. albicans antibodies is to be developed.Abbreviations GYE
glucose-yeast extract agar 相似文献